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After washing in PBS, primary antibodies (IgM-PE BioLegend 406507, IgD-APC BioLegend 405713, MARCO Santa Cruz sc-65353/ anti-rat PE Abcam ab97058 and Siglec-1 Biolegend 142417) diluted in PBS 10% FCS were incubated overnight at 4 C

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Trivedi et al

This was done to assess whether the concentration dependent effects of exogenous EGT on the stored broccoli were correlated to changes in ascorbate redox and glutathione redox states ( Figure 8 )