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Cleaning-in Place, CIP (1) To remove precipitated or denatured proteins, wash the column with 2 bed volumes of 6 M guanidine hydrochloride followed by 5 bed volumes of PBS (pH 7.4) washed

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The concern isnt just that youll develop antibodies against the peptide itselfits that those antibodies might cross-react with your own endogenous proteins

Jzwiak, M., Bauer, M., Kamysz, W., & Kleczkowska, P