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The fish were anesthetized with MS-222 (0.003%) (SigmaAldrich, Saint Louis, USA) prior to immersion in epinephrine (10 mg/ml) (SigmaAldrich) for a period of 15 min to facilitate melanosome contraction

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Hormone changes can also cause normal variations in discharge during the menstrual cycle, such as thicker, egg-white-like mucus at one time and whiter discharge at another

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Every approach has advantages and drawbacks