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Aluminum crosses the blood brain barrier
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The sections were developed with chromogenic agent A, B, and C using DAB kit (Sigma-Aldrich) for 6 min, stained with hematoxylin for 30 s, dehydrated in ascending series of alcohol (70%, 80%, 90%, and 95% ethanol and anhydrous in sequence) for 2 min, immersed twice in xylene for 5 min and sealed with neutral resin before observation and counting under an upright microscope (BX63, Olympus Optical Co., Ltd., Tokyo, Japan)
Clark, R G, and I C Robinson
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