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The bound protein was eluted by elution buffer (50 mM TrisHCl pH 8.0, 300 mM NaCl and 250 mM imidazole) and loaded onto a Superdex 75 gel filtration column equilibrated with ITC buffer (20 mM Na-Mes pH 5.5 and 150 mM NaCl)
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or breastfeeding
R.TerrillA
Pricing can shift depending on the dose
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