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For a 0.5 mg/mL solution, add 10 mL

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Additionally, certain medications including anticonvulsants and nucleoside analogues used in the treatment of HIV infection, may produce a secondary L-carnitine deficiency

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Consequently, HILIC method development and validation require thorough risk assessment during method development to identify and minimize sources of variability and ensure robust methods suitable for GMP settings (which is entirely possible!)