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doi: 10.1016/j.ynpai.2022.100091 44
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BMC Genomics 18:266
Knockdown of ZDHHC4 in cardiomyocytes reversed the increase in CD36 palmitoylation induced by TGR5 deletion, suggesting that the TGR5-ZDHHC4 pathway is a key target for intervening in lipid metabolism in diabetic cardiomyopathy (185)
The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2
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