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The mixture of hA3A-eBE3-Y130F mRNA (100 ng/L) and Trim37- sgRNA-stop (100 ng/L) was diluted in RNase-free water, centrifuged at 13,400 g for 10 min at 4 C, and injected into the cytoplasm of zygotes ( mTmG Blimp1-T2A-Cre or B6D2F1 Nanog-EGFP ) using a micromanipulator (Olympus, Tokyo, Japan) and a FemtoJet microinjector (Eppendorf)
Across all groups, ease of use scores consistently fell within the easy to neutral range, while satisfaction scores were in the satisfied to neutral range (Online Resource 2)
Description Glutathione (-L-glutamyl-L-cysteinyl-glycine) is the most abundant intracellular antioxidant tripeptide in mammalian cells, present at millimolar concentrations in the cytosol, mitochondria, and nucleus
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Parkin ubiquitinates outer mitochondrial membrane proteins, including voltage-dependent anion channel 1 (VDAC1) and mitofusins (Mfn1 and Mfn2), thereby initiating mitophagy
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