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Following the dehydration steps described above, slides were washed three to five times in RT diH 2 0 and then for 10 s in hot diH 2 0 to acclimate and subsequently incubated in hot 1 TRR (322000, ACD) for 5 min according to the manufacturers instructions
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Obstructive Sleep Apnea and Testosterone Therapy
doi:10.1159/000354678
Cys 2 ), cystine-free (Cys 2 -free), cystine-free/L-NAC-supplemented (400 M), cystine-free/D-NAC-supplemented (400 M) medium, untreated or treated with BSO (100 M) for 72 h, and relative cell numbers were determined (n = 4 technical replicates)