PubMed, Yuan, Shuai, Amy M
Acetyl-L-carnitine supplementation differently influences nutrient partitioning, serum leptin concentration and skeletal muscle mitochondrial respiration 16
GCLC catalyzes the rate-limiting step of GSH synthesis
Cells were then permeabilized with 0.1% Triton-X-100 (Sigma-Aldrich) for 5 minutes, then incubated for 1 h with a saturation solution (10% of goat serum (Sigma-Aldrich), 3% of human serum (Sigma-Aldrich), 0.1% Triton-X-100 (Sigma-Aldrich), 1% BSA (Euromedex), 0.05% Tween 20 (Euromedex) in PBS
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