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Briefly, the brain homogenate was diluted 1:20 (v:v) with ice-cold Lockes buffer (154 mM NaCcl, 5.6 mM KCl, 3.6 mM NaHCO3, 2.0 mM CaCl2, 10 mM D-glucose and 5 mM HEPES, pH 7.4) to obtain a concentration of 10 mg tissue/ml

Lack of NPGPx causes elevation in ROS and promotion of adipogenesis through ROS-dependent dimerization of protein kinase A regulatory subunits and activation of C/EBP [105]

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The cells were exposed for various times, after which the media was removed and the cells were washed with warm PBS and lysed by brief sonication in fresh PBS

These results demonstrate that PGC depletion in Trim37 knockout mice is most likely PGC autonomous