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Measurement of ALB, AST, and ALT To quantify ALB, ALT, and AST, organoid culture supernatants were collected after 24 hours, and the concentrations of secreted ALB, ALT, and AST were measured using Human Albumin (Bethyl Laboratories, Montgomery, TX, USA), ALT (Abcam, Cambridge, UK), and AST (Abcam) ELISA kits according to the manufacturers instructions

After weekly subcutaneous injection, EX was slowly released from the microspheres by diffusion and microsphere rupture and reached a stable plasma concentration about 6-8 weeks after treatment (114)

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Systems were equilibrated in isothermal-isobaric conditions (NPT) using the Berendsen barostat 51 with a target pressure of 1 atm, the Langevin thermostat 52 with a target temperature of 310 K, along with a low damping factor of 1 ps 1 and an integration time step of 2 fs

Injectable tirzepatide: Trials showed approximately 22.5% average weight loss at the highest doses

annual testing recommended Schedule quick follow-ups with Dr