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The surface was washed between samples with 25 mM NaOH for 4 s followed by equilibration with running buffer

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TB-500 sits at the bioactive Ac-SDKP tetrapeptide fragment level, and the three supporting components at 10mg each provide concentration parity for pathway interaction studies without the dominant component being diluted below practical research thresholds

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Since there was no significant difference of the mRNA level of GCS-HS and GS between control and proline group at any time points, it can be assumed that the activity of GCS in proline group might be upregulated by the upregulation of regulatory subunit of GCS, leading to the significant upregluation of total GSH from 6 h to 12 h

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