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New vectors were confirmed by insert sequencing
The primary antibodies used included anti-ZO-1 (Abcam, ab307799, 1:5000), anti-Occludin (Abcam, ab216327, 1:5000), anti-ACSL4 (Abcam, ab155282, 1:10000), anti-TFR1 (ABclonal, A5865, 1:2000), anti-SLC7 A11 (Abcam, ab175186, 1:5000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-VDAC2 (Proteintech, 116631-AP, 1:5000), anti-VDAC3 (Proteintech, 552601-AP, 1:5000), -tubulin (Proteintech, 100681-AP, 1:5000), and GAPDH (Proteintech, 104941-AP, 1:20000)
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Typical protocol structure: Dose: 100-200mcg based on individual response Frequency: 3-5 times per week Timing: 1-3 hours before bed (experiment to find your optimal window) Cycle length: 4-8 weeks Break period: 2-4 weeks between cycles The frequency debate generates significant discussion
Alba Therapeutics Announces Positive Results of Phase IIb Trial in Celiac Disease