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10.1016/j.biochi.2006.10.014 Biochimie 19 ChenL.YangH

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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

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1- surface charge-driven interaction: The positive zeta potential of PTX-CSNPs (+ 32.77 mV) promotes electrostatic binding to negatively charged components of the glomerular filtration barrier, including heparan sulfate proteoglycans in the glomerular basement membrane and the glycocalyx of proximal tubular cells, thereby prolonging renal retention

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Argelles, J

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