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To obtain the necessary number of animals for the experiment, eight Wistar females were mated with eight Wistar males (1:1, individual cages) after checking the estrous cycle adapted from Marcondes and collaborators (2002)
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& Schmid, H
It is reported that a high-fat, high-carbohydrate diet structure, irregular lifestyle, and low physical activity are the main detrimental factors for MetS (20)
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