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For optimal long-term preservation, materials may be stored frozen
Rac1 normally cycles between the GTP and GDP bound states, depending on the redox state of Rac1
Redox [edit] Thiols, especially in the presence of base, are readily oxidized by reagents such as bromine and iodine to give an organic disulfide (RSSR)
Levels decrease with age
Additionally, the time required to complete the enzymatic activity measurements was prolonged, and the method needed to be more easily adaptable to microplate readers due to the necessity of protein precipitation
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