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Lentivirus production and cell transduction Lentiviral particles were produced as previously described [32] employing, psPAX2 and pMD2G-VSVG plasmids [provided by D

Sangsuwan W, Asawanonda P

however, the clinical significance and durability of these effects remain under investigation

Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively

Drinking one to two cups of coffee should do the trick

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