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Primers were designed to fit in resulting vector, pJB-HTS upstream of the hexa-histadine tag (5/phos/CCATGGGCAGCAGCCATCATCAT), and downstream (AGCTGGAATTCCTAGTTATTGCTCAGCGGTGGC) (Integrated DNA technologies) of the spacer yielding a fragment containing a phosphorylated 5 end, an initiation codon, a hexa-histadine tag, a thrombin cleavable sequence, a spacer, a termination codon, and an EcoRI site
doi: 10.4049/jimmunol.1900658 118 FuGGuyCSChapmanNMPalaciosGWeiJZhouPet al
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Muscle & Nerve: Official Journal of the American Association of Electrodiagnostic Medicine, 10 (7), 577-598
