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Oxidized to reduced GSH ratio was determined using GSSG/GSH detection kit (Enzo Diagnostics, New York, NY, USA) as described in the manufacturers guidelines

Therapy: administer for 5-7 consecutive days

10.1016/j.cell.2017.02.031 Cell 5 BorodkinaA

At elevated doses, isolated reports document nausea and gastrointestinal discomfort, though frequency, severity, and dose-dependency remain uncharacterized in controlled studies

Rapid weight loss changes estrogen levels, and the hormonal shifts that accompany significant fat loss can temporarily alter cycle timing, flow, and symptoms

A change could make things safer and secure the treatment in the long run