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Stable CHOFlpIn expression cell lines were selected using 600 g/ml hygromyocin B, and maintained in DMEM supplemented with 5% (V/V) FBS (Invitrogen) at 37C in 5% CO 2

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Alcohol wipes 2-4ml syringe depending upon injection quantity

Proceedings of the National Academy of Sciences of the United States of America 88 , 93609364 (1991)