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Preparation of cell lysates Cells were sonicated three times for 10 s in ice-cold immunoprecipitation/kinase buffer (50 mM Hepes, pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 10% glycerol, 0.1% Tween 20, 0.1 mM PMSF, 10 g/ml leupeptin, 10 g/ml aprotinin, 1 mM sodium fluoride, 10 mM sodium -glycerophosphate, and 0.1 mM sodium vanadate) and incubated for another 30 min on ice

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Improves Brain Function Vitamin B12 is required by the body to make red blood cells, which carry oxygen to the brain and other organs

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Patients may interrupt treatment due to side effects, supply shortages, financial constraints, or medical procedures

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