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Table 1 Abbreviations and respective meanings thereof Fmoc 9-fluorenylmethoxycarbonyl 2-CTC-Resin 2-chlorotrityl chloride resin tBu tert-butyl OtBu tert-butoxy Alloc allyloxycarbonyl DCM dichloromethane DBLK 20% hexahydropyridine/DMF solution DIPEA N,N- diisopropylethylamine HOBt 1-hydroxybenzotriazole HOAt 1-hydroxy-7-azobenzotriazole PyBOP benzotriazol-1-yl-oxytripyrrolidinyl hexafluorophosphate DMSO dimethyl sulfoxide HATU O -(7-azobenzotriazole)-N,N,N',N'-tetramethyluronium hexafluorophosphate HPLC High Efficiency Liquid Chromatography DMF N,N - the raw materials and reagents used in the preparation method of Semaglutide provided by the present invention are all commercially available

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