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Cell Metab (2011) 13(5):51726

After stabilization, leak respiration supported primarily by electron flow through complex I of the respiratory chain was evaluated by adding the substrates malate (0.5 mM), pyruvate (5 mM), and glutamate (10 mM)

If your calculated dose requires a volume larger than your syringe can hold, you can split it into two injections given at the same time in different sites

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