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To assess the effect of L-Cn on T cell subsets, PBMCs or purified T cells were stimulated with or without L-Cn (20 mg/mL) in the presence of anti-CD3/CD28 (5 g/mL) for 72 h and restimulated with or without L-Cn for another 6 h in the presence of PMA (50 ng/mL), ionomycin (0.75 g/mL), and BFA (1 g/mL)

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