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The hippocampus was dissected out on ice and immediately homogenized in the lysis buffer with 1% phenylmethylsulfonyl fluoride and 1 PhosSTOP phosphatase inhibitor cocktail (Roche Applied Science, Penzberg, Germany)
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As an electrophilic chemical, ITCs might react covalently with the thiol groups of (proteins and) glutathione in plants, similar to what has been demonstrated in different in vitro systems 38,77,78 as well as in insects feeding on GSL or ITC diets 79,80
Obesity continues to be a global health concern, with its prevalence steadily increasing over the years
The chemical stays within the tree for two years, and treatment in the spring is important due to EABs lifecycle
Many research peptide suppliers now provide Certificates of Analysis from these independent labs
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