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Olympus, Tokyo, Japan) and continuously superfused at a flow speed of 1 mL/min with a bath solution containing 140 mM NaCl, 5 mM KCl, 1 mM CaCl 2 , 2 mM MgCl 2 , 10 mM HEPES, and 10 mM glucose, adjusted to pH 7.4 with NaOH

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For those with low strength, further assessment of muscle mass (such as bioelectrical impedance analysis and dual-energy X-ray absorptiometry) is conducted, and the severity is defined by physical manifestations (such as walking speed and the Brief Physical Condition Scale) [2, 3]

Upon release, Nrf2 translocates to the nucleus and binds to the antioxidant response element (ARE) located in the promoter of genes coding for antioxidant/detoxifying enzymes and scavengers