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The water molecule activated by Glu235 acts as a primary proton acceptor by abstracting a proton from the hydroxyl group of an incoming phenolic substrate, which is subsequently transferred to the carboxylic group of the conserved glutamic acid 59
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This concentration has been validated to maintain sterility over the 28-day multi-use period when proper aseptic withdrawal technique is followed in laboratory settings
Irfan HM, Asmawi MZ, Khan NA, Sadikun A, Mordi MN
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