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The animals were placed in an induction chamber with an oxygen-enriched environment, with the initial induction concentration set at 4%5% isoflurane, until deep anesthesia signs such as loss of the righting reflex appeared, after which maintenance was initiated

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Moreover, the limitations of animal models in translating findings to human addiction underscore the need for caution, since results may not always directly apply to humans

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Thus non-specific transport into the brain is generally limited to passive diffusion across the apical and basolateral membranes of the hBMECs that form the lumen of the cerebrovasculature