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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development

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TB500 is a synthetic fragment of thymosin beta-4, a highly conserved protein that binds to actin and participates in cytoskeletal organization, cell migration, and intracellular transport

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J Biol Chem 284, 90599065, 10.1074/jbc.M809608200 (2009)