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For the mitochondrial OCR assays, mitochondria were plated on Seahorse Biosciences 96-well culture plates (5 g of protein per well) and centrifuged for 20 min at 2,000 g at 4 C to adhere mitochondria to the base of wells

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Additionally, the expression of glutathione metabolism-associated enzymes was measured in C

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J Biol Chem 291(3):12031220 Martn-Escolano R et al (2021a) Selenium derivatives as promising therapy for chagas disease: in vitro and in vivo studies