Furthermore, the protein content was determined in lysed PBMC to generate a normalization factor, using a bicinchoninic acid assay (BC Assay, Uptima, Interchim, Montluon, France)
So, thats the big issue
Acknowledgement This project was funded in part with an integrated internal competitive grant from the College of Agriculture and Life Sciences at Virginia Tech
Curcumin reduces the production of pro-inflammatory cytokines, such as TNF- and IL-6, which are central to the pathophysiology of diabetes and obesity (Hussain et al., 2022)
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