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After 24, 48 or 72 h exposure to experimental conditions, the conditioned culture medium (supernatant) was collected, and adherent cells were detached with 0.05% trypsin-EDTA

However, in the presence of 20% human plasma, a 100-fold rightward curve shift was observed for the lipidated compounds, but not the conjugates and the GLP-1 analogue, indicating that MK-801 and inactive MK-801 do not associate strongly with HSA (Fig

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