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J Perinat Med 1995;23:477-85

the pET24 plasmid vector was isolated from overnight grown E.coli ToplO-pET24 culture

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However, very low-calorie diets (VLCDs) typically defined as fewer than 800 kcal per day can place significant physiological stress on the body and should only be undertaken under medical supervision, in line with NICE guideline CG189 (Obesity: identification, assessment and management)

Microbiome 9 , 34 (2021)