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This observed process, lasting typically 4-8 weeks in some research, is an important consideration when evaluating its effects
To measure PIP 3 , FoB were stimulated with 5 g/mL anti-IgM (Jackson Immunoresearch 715-006-020) for the indicated time points, then fixed in 2% FA and permeabilized with 0.1% saponin
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After 21 days of culture, EHM were subjected to isometric force measurements as previously described ( Life Cell Imaging of Mitochondria Cells were seeded onto glass bottom imaging dishes (Zell-Kontakt) using growth conditions stated above
Such responses usually resolve within the first two weeks
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