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The gut microbiota-derived metabolite trimethylamine N-oxide is elevated in Alzheimers disease
Total RNA was isolated from fresh-frozen pancreatic or cells using Trizol reagents and cDNA was prepared using Sprint qPCR RT kit (Mei5bio, MF949-01) and was subjected to quantitative PCR using SYBR qPCR Mix (Mei5bio, MF947) according to the protocols provided by manufacturers
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