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Quantification of nerve growth factor (NGF) levels was performed using a commercially available Rat NGF ELISA kit (Elabscience , Catalog No: E-EL-R0652, Houston, TX, USA), following the manufacturers protocol.The specificity was validated by the manufacturer, with no significant cross-reactivity or interference observed

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Complex neurochemical signals, hormones, and metabolic issues can make it very hard to take in fewer calories, Varney said

The transcripts generated from these regions, known as ncRNAs, represent a class of RNA molecules with critical regulatory functions, primarily encompassing microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) 117