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RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)

Avoid large milk servings immediately after your weekly injection, when GLP-1 levels are peak and nausea risk is highest

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At this point youve been on the medication for two months and your body is adapting more efficiently to dose changes