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Detection of ADP from Cp1B assay The reaction mixtures (200 l) in 100 mM Tris-HCl buffer (pH 8.0) contained 0.25 M Cp1B, 10 mM ATP, 12 mM MgCl 2 , 300 M reduced nicotinamide adenine dinucleotide (NADH), 500 M phosphoenolpyruvic acid (PEP), 41 U per ml pyruvate kinase (PK
46 The location of application depends on the product
Reconstituted solution: Refrigerate at 4 C (39 F)
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Steady-state kinetic analysis showed that Arabidopsis GS uses a random Ter-reactant mechanism with binding of either ATP or -glutamylcysteine preferred first and addition of glycine last (Jez and Cahoon, 2004)
The most commonly reported side effects are mild and temporary: Nausea A brief headache Slight dizziness Minor skin sensitivity at the IV site These typically resolve within a few hours without intervention
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