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Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81

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Common causes include: Reduced dietary intake People following vegan or strict vegetarian diets can be at higher risk if theyre not using B12-fortified foods or supplements