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Typically, treatment will be once each week for 2-6 weeks
Optimization of Agrobacterium-mediated transformation through genotype-specific modification of explant type, bacterial strain, virulence induction conditions, co-cultivation duration, and selection regimes can significantly enhance transformation success
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Draw slightly more than needed, then push back to exact volume to ensure precision
Muscle Nerve 62 , 321326 (2020)
Brain sections at bregma1 mm ( n = 4 per group) were deparaffinized and treated with xylene and decreasing graded ethanol to distilled water for hydration
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