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The samples were then centrifuged at 18 000 g at 4 C for 15 min, and 20 l of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay
PMID: 21447641 Sekhar RV, et al
While it is clear that oxidative stress impairs sperm function and increases the risk of infertility, there is growing evidence to suggest that oxidative damage to sperm DNA may have long-term consequences for offspring, including increased susceptibility to genetic disorders and developmental abnormalities
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