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When the density reached about 70%, cells were pre-treated with different concentrations of HMSeBA (300, 600, and 1200 nM) for 24 h, attended by co-treatment with H 2 O 2 (0.1, 0.2, and 0.4 mM) (Chron chemicals, Chengdu, China) for another 2 h
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Importantly, these subpopulations were also identified in vivo transplants, where the proportion of mature 1 cells was higher than in vitro cultures, underscoring the role of the physiological microenvironment in maintaining the mature -cell phenotype (Kang et al., 2023)
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