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Blocking was performed with PBS 1 0.5% BSA 0.1% saponin (w/v/v), between incubations, cells were rinsed three times with PBS 1, for 5 min

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This isn't speculation

Benzyl alcohol has been linked to a fatal "gasping syndrome" in newborns, so only preservative-free sterile water for injection should be used in that group

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