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Short answer: one GLOW dose is 10 units 0.10 mL on a U-100 insulin syringe, from a 70 mg vial reconstituted with 3 mL of bacteriostatic water

Another concept is to measure B12 bound to the plasma protein transcobalamin (holoTC, active B12) before and after administration of three high physiological doses of B12 for two days

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Ashabi G, Khalaj L, Khodagholi F, Goudarzvand M, Sarkaki A: Pre-treatment with metformin activates Nrf2 antioxidant pathways and inhibits inflammatory responses through induction of AMPK after transient global cerebral ischemia

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Protection from light is particularly important if vials are stored in transparent refrigerators