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Plasma was isolated by centrifugation at 17,000 g for 5 min at 4 C and diluted 1:10 in 50% (v/v) glycerol in PBS (pH 7.4) (Sigma-Aldrich) for storage at 20 C until analysis

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Each pen is pre-filled with four doses of semaglutide, so theres no complicated drawing up procedure

Patients are never contacted about trials without consent