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1.8 and 2.3 during sensor calibration over time in both species ( Fig
Lipids in liver tissue (50 mg) were extracted by chloroform:methanol solution (2/1, v/v), and total cholesterol levels were determined by enzymatic kit as previously described 43
This conservative approach allows for assessment of individual tolerance before increasing to the 2-5mg range used in more intensive protocols
Front Aging Neurosci 13:654931
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