This feature and its negative charge at physiological pH imply the existence of specific carriers to import GSH from the cytosol to the mitochondrial matrix, where it plays a key role in defense against respiration-induced reactive oxygen species and in the detoxification of lipid hydroperoxides and electrophiles
The research protocol received ethical clearance from the Institutional Review Board of the First Affiliated Hospital of Nanjing Medical University
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(Oxidative stress and diseases)
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Dulbeccos modified Eagles medium (DMEM), fetal bovine serum, random primers and SYBR safe DNA gel stain were supplied by Invitrogen, Life Technologies (USA)