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First, we examined the expression profiles of Ser-GST genes in the context of a developmental time-course in Arabidopsis (Figure 3), using the AtGenExpress Developmental Set (Schmid et al., 2005)

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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature