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Hyperactivation of mTOR promotes macrophage proliferation, inhibits apoptosis, and drives metabolic reprogramming, thereby supporting macrophage survival and granuloma persistence (46, 47)

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Required Equipment and Materials Microplate reader (with 405 nm filter or 415 nm filter) 96-well microplate 20 l, 200 l and multi-channel pipettes Incubator (37C) 5-Sulfosalicylic acid (SSA) Precaution Use the reagents in the kit after the reagents temperatures are equilibrated to the room temperature

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